Diyala Journal for Pure Science DJPS

P- ISSN:2222-8373, E-ISSN:2518-9255

Volume 16, Issue 1 , January  2020

Estimation, Partial Purification of Lipoxygenase and Estimate GGT in the Blood Patients with Prostate Cancer

Muslim Abbas Abd Al-Adlee and Nadia Ahmed Salih Al-Guburi

Year: 2020, Volume: 16, Issue: 1

Pages: 88-101 , DOI:  https://dx.doi.org/10.24237/djps.16.01.515B

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Abstract

The goal of study was to evaluate the activity, partial purification of LOX enzyme and the evaluation of GGT activity in serum of prostate cancer patients in Baghdad after having been diagnosed with prostate cancer through CT scans or MRI and a biopsy in the Oncology hospital for the period (19-02-2018 - 28/02/2019). The study included 50 samples of men with prostate cancer. Also, the study comprise (50) men samples (control) As a comparative group. The results showed that serum LOX activity was significantly increased in prostate cancer patients compared to the control. The serum enzyme LOX of prostate cancer was purified by following step: precipitate serum protein (40% ammonium sulfate), dialysed against a buffer solution, The gel filtration technique was use to separation the enzyme from other proteins (Sephadex G.100), we can obtained one peak in this step  with purity (6.732) fold and yield (27.77 %). The study also included determination the molecular weight of enzyme by electrophoresis technique with polyacrylamide gel, its molecular weight was (70) KD.

Also, the results showed a significant increase GGT activity in patients (P ≤ 0.000) compared to control. These blood samples were obtained from the Oncology Hospital - city Medicine - Baghdad.

Keywords: lipoxygenase (LOX), lox enzyme purification, prostate cancer, gamma - glutamyl transferase (GGT).