Diyala Journal for Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal for Pure Science DJPS
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 17, Issue 2 , April 2021
Study of pvd A Gene Sequencing from Pseudomonas aeruginosa Isolated from Burn Infections
Arwa M. Abdullah Al-Shuwaikh, Rana M. Abdullah Al-Shwaikh and Abbas Falih Al-arnawtee
Year: 2021, Volume: 17, Issue: 2
Pages: 1-12 , DOI: https://dx.doi.org/10.24237/djps.17.02.542C
Pseudomonas aeruginosa possesses two types of iron transport systems and one of them is Pyoverdine, which is fluorescent under the UV light and encoded by the PvdA gene. This pigment is great importance in iron deficiency or its loss affects to cell division and stops the DNA manufacturing process. The other type of pigment is Pyochelin, and that both types are formed during infection with P. aeruginosa and tissue invasion in the host's body. The DNA sequence was analyzed for the pvdA gene in P. aeruginosa that isolated from the burns. The results of this study showed that 15 isolates of the P. aeruginosa from 23 isolates have pvd A gene at 65.22%, while 8 isolates did not have the pvdA gene at a rate of 34.78 %, with molecular weight of 1281 base pairs. Also, the analysis of pvd A gene sequencing showed that there are 21 genetic mutations. Include insertion, deletion and replaced.
Keywords: Pseudomonas aeruginosa, pvd A gene, Mutations.