Diyala Journal for Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal for Pure Science DJPS
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 17, Issue 2 , April 2021
Detection of Common Bacterial Contaminants of Locally Produced Al-Arab Soft Cheese in Diyala Province
Walaa Mahmood Mohamed*, Shaymaa Jabbar Hasson, Hanaa kareem Ali Alshammary, Adasa Khaleel Ibrahim and Dena Mohamed Khudaier
Year: 2021, Volume: 17, Issue: 2
Pages: 38-51 , DOI: https://dx.doi.org/10.24237/djps.17.02.546E
One of the common types of cheese manufactured in Iraq is Al-arab cheese. The reason for this is due to high proportion of protein ,texture and price. However, the conventional way that used to produce and sell it affords many opportunities for microbial contamination. To determine the hygiene quality of Al-arab cheese production in Diyala, 75 samples were collected randomly from (Baqubah, Dali Abbas, Khalis, Jalawlaa, Khan bani saad) towns. All samples were cultured on nutrient agar and positive culture was subjected to antibiotics susceptibility test using (Trimethoprim, Tetracycline Vancomycin, Cloxacilin, and Amoxicillin). Staphylococcus aureus recovered from Baqubah, Khalis, Khan bani saad, and Jalawlaa respectively (80% ,60%, 40% and 20%). Escherichia coli was the predominant in samples gathered from Dali abbas, Baqubah, Khalis, and Jalawlaa respectively (80%, 60%,60% and 20%). pseudomonas spp. Recovered from Baqubah, Khalis, and Dali abbas respectively (40%, 10% and 40%). Salmonella spp. was recorded the lowest percentage of contamination (10%) in the samples were collected from Al-Khalis
Trimethoprim was effective against S. aureus and Klebsiella with mean inhibitory zone diameter 34mm and 36,7mm respectively while the inhibitory zone diameter for E. coli was (30.3) toward tetracycline. Amoxicillin, Cloxacilin, and Vancomycin were not effective against Staphylococcus aureus, E. coli, Klebsiella spp.
The high frequency of isolation for S.aureus , E. coli, Klebsiella, Salmonella spp and pseudomonas spp from Al-arab cheese indicated that both heat treatment and storage temperature not sufficient to ensure consumer safety. Adequate heat treatment for superior raw milk quality and low initial contamination level with bacteria during Al-arab cheese processing and storage are regarded as the important steps for reduction contamination in locally produced products.
Keywords: Soft cheese, Staph aureus, E. coli, Klebsiella sp.