Diyala Journal for Pure Science
Scientific Refereed Journal Published By College of Science - University of Diyala
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Diyala Journal for Pure Science DJPS
P- ISSN:2222-8373, E-ISSN:2518-9255
Volume 17, Issue 3 , July 2021
Lateral Flow Immunoassay Based Colloidal Gold Nanoparticles for the Detection of Organophosphorous Pesticide (Propanoic) in Cow’s Milk
Azhar M. Haleem, Israa M. Haleem and Ruaa H. Abass
Year: 2021, Volume: 17, Issue: 3
Pages: 1-13 , DOI: https://dx.doi.org/10.24237/djps.17.03.18-555-ICPS
Simple, inexpensive, and high sensitive Lateral Flow Immunoassay (LFI) based colloidal gold nanoparticles antigen-coated as the label was designed for the fast determination of organophosphorus pesticide in cow’s milk samples. The developed LFI detection strip consisted of four structural zones start from the sample pad, conjugate pad, detection zone, and finally absorbent pad. Immune-sensitive zone was synthesized and functionalized with Propanoic Hapten–OVA, Goat monoclonal-anti-Propanoic antibodies were produced. Propionic -cationized ovalbumin-HPR conjugates were fabricated and identified. The antibody optimal conditions were set and the influence of pH on the analytical properties of the antibody-based gold nanoparticles was also investigated. The detection limit of the LFI for Propanoic standard into milk samples were 0.5 and 100 ng/mL, respectively. The test development time for the LFI was less than 5 min, suitable for fast on-site measuring of Propanoic.
Keywords: Pesticides, lateral flow immuno-assay, organophosphorous, cow milk, gold nanoparticles.